PT  - JOURNAL ARTICLE
AU  - Palmowski, Michael J.
AU  - Lopes, Luciene
AU  - Ikeda, Yasuhiro
AU  - Salio, Mariolina
AU  - Cerundolo, Vincenzo
AU  - Collins, Mary K.
TI  - Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response
AID  - 10.4049/jimmunol.172.3.1582
DP  - 2004 Feb 01
TA  - The Journal of Immunology
PG  - 1582--1587
VI  - 172
IP  - 3
4099  - http://www.jimmunol.org/content/172/3/1582.short
4100  - http://www.jimmunol.org/content/172/3/1582.full
SO  - J. Immunol.2004 Feb 01; 172
AB  - Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1157–165-specific CD8+ cells, detected ex vivo with an A2/H-2Kb chimeric class I tetramer. These NY-ESO-1157–165-specific CD8+ cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1157–165 peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1157–165-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy.