PT  - JOURNAL ARTICLE
AU  - Kelly, Janice M.
AU  - Takeda, Kazuyoshi
AU  - Darcy, Phillip K.
AU  - Yagita, Hideo
AU  - Smyth, Mark J.
TI  - A Role for IFN-γ in Primary and Secondary Immunity Generated by NK Cell-Sensitive Tumor-Expressing CD80 In Vivo
AID  - 10.4049/jimmunol.168.9.4472
DP  - 2002 May 01
TA  - The Journal of Immunology
PG  - 4472--4479
VI  - 168
IP  - 9
4099  - http://www.jimmunol.org/content/168/9/4472.short
4100  - http://www.jimmunol.org/content/168/9/4472.full
SO  - J. Immunol.2002 May 01; 168
AB  - We have investigated the primary and secondary immunity generated in vivo by a MHC class I-deficient tumor cell line that expressed CD80 (B7-1). CD80 expression enhanced primary NK cell-mediated tumor rejection in vivo and T cell immunity against secondary tumor challenge. CD80 expression enhanced primary NK cell-mediated tumor rejection, and both NK cell perforin and IFN-γ activity were critical for the rejection of MHC class I-deficient RMA-S-CD80 tumor cells. This primary rejection process stimulated the subsequent development of specific CTL and Th1 responses against Ags expressed by the MHC class I-deficient RMA-S tumor cells. The development of effective secondary T cell immunity could be elicited by irradiated RMA-S-CD80 tumor cells and was dependent upon NK cells and IFN-γ in the priming response. Our findings demonstrate a key role for IFN-γ in innate and adaptive immunity triggered by CD80 expression on tumor cells.