RT Journal Article
SR Electronic
T1 A Dominant Role for Fcγ Receptors in Antibody-Dependent Corneal Inflammation
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 919
OP 925
DO 10.4049/jimmunol.167.2.919
VO 167
IS 2
A1 Hall, Laurie R.
A1 Diaconu, Eugenia
A1 Pearlman, Eric
YR 2001
UL http://www.jimmunol.org/content/167/2/919.abstract
AB Although production of specific Ab is a critical element of host defense, the presence of Ab in tissues leads to formation of immune complexes, which can trigger a type III Arthus reaction. Our studies on a mouse model of river blindness showed that Ab production is essential for recruitment of neutrophils and eosinophils to the cornea and for development of corneal opacification. In the current study, we determined the relative contribution of complement and FcγR interactions in triggering immune complex-mediated corneal disease. FcγR−/− mice, C3−/− mice, and immunocompetent control (B6/129Sj) mice were immunized s.c. and injected intrastromally with Onchocerca volvulus Ags. Slit lamp examination showed that control mice, C3−/− mice, and control mice injected with cobra venom factor developed pronounced corneal opacification, whereas corneas of FcγR−/− mice remained completely clear. Furthermore, recruitment of neutrophils and eosinophils to the corneal stroma was significantly impaired in FcγR−/− mice, but not in C3−/− mice or cobra venom factor-treated mice. We therefore conclude that FcγR-mediated cell activation, rather than complement activation, is the dominant pathway of immune complex disease in the cornea. These findings demonstrate a novel role for FcγR interactions in mediating ocular inflammation.