RT Journal Article SR Electronic T1 Human Polymorphonuclear Leukocytes Produce IL-12, TNF-α, and the Chemokines Macrophage-Inflammatory Protein-1α and -1β in Response to Toxoplasma gondii Antigens JF The Journal of Immunology JO J. Immunol. FD American Association of Immunologists SP 7369 OP 7375 VO 162 IS 12 A1 Bliss, Susan K. A1 Marshall, Anthony J. A1 Zhang, Yin A1 Denkers, Eric Y. YR 1999 UL http://www.jimmunol.org/content/162/12/7369.abstract AB The induction of a type 1 inflammatory cytokine response is a key event in the initiation of immunity to Toxoplasma gondii. Because polymorphonuclear leukocytes rapidly respond to infection by exiting the peripheral blood and accumulating at a site of infection, we sought to determine whether these cells produce cytokines in response to T. gondii. When human peripheral blood neutrophils were stimulated with parasite Ag, they produced both IL-12 (p70) and TNF-α. Similarly, up-regulated expression of macrophage-inflammatory protein-1α (MIP-1α) and MIP-1β gene transcripts was induced. Kinetic analysis of IL-12 and TNF-α production revealed distinct patterns following stimulation by T. gondii or LPS. Exogenous TNF-α alone also provided a potent stimulus of MIP-1α and MIP-1β expression, and when neutralizing anti-TNF-α antiserum was included in cultures of parasite-stimulated cells, expression of these CC-family chemokines was partially blocked. These results establish that T. gondii possesses the ability of driving neutrophil proinflammatory cytokine production, and they suggest that parasite-induced MIP-1α and MIP-1β partly results from autocrine stimulation through TNF-α.