RT Journal Article
SR Electronic
T1 Integrin-mediated intracellular Ca2+ signaling in Jurkat T lymphocytes.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 1618
OP 1627
VO 158
IS 4
A1 Weismann, M
A1 Guse, A H
A1 Sorokin, L
A1 Bröker, B
A1 Frieser, M
A1 Hallmann, R
A1 Mayr, G W
YR 1997
UL http://www.jimmunol.org/content/158/4/1618.abstract
AB T lymphocytes interact with components of the extracellular matrix after transendothelial migration on their way to sites of inflammation. To characterize the molecular basis of the interaction between T lymphocytes with different extracellular matrix proteins, we investigated the role of intracellular Ca2+ as a signal mediating such interactions and identified the cell surface integrins involved in this process. When Jurkat T lymphocytes loaded with the calcium-sensitive fluorescent dye fura-2 were placed on coverslips coated with human fibronectin, human collagen types I, IV, and VI, human tenascin, human laminin I, or mouse laminin I, an elevation in intracellular Ca2+ concentration was observed. In contrast, contact of the Jurkat T lymphocytes with vitronectin and thrombospondin did not induce Ca2+ signals in more cells as compared with control measurements in which cells were in contact with only BSA or polylysine. Furthermore, the percentage of Jurkat T lymphocytes responding with Ca2+ signals to collagen types I and IV, fibronectin, and laminin I was completely reduced to levels observed on BSA or polylysine when the cells were pretreated with specific anti-integrin Abs, suggesting a role for cell surface integrins as mediators of cell matrix-induced intracellular Ca2+ signaling. Similar results were obtained with peripheral human T lymphocytes activated by phytohemagglutinin.