PT  - JOURNAL ARTICLE
AU  - Zerrahn, J
AU  - Utermöhlen, O
AU  - Warnecke, G
AU  - Deppert, W
AU  - Lehmann-Grube, F
TI  - Protective immunity in BALB/c mice against the simian virus 40-induced mKSA tumor resulting from injection of recombinant large T antigen. Requirement of CD8+ T lymphocytes.
DP  - 1996 May 15
TA  - The Journal of Immunology
PG  - 3919--3924
VI  - 156
IP  - 10
4099  - http://www.jimmunol.org/content/156/10/3919.short
4100  - http://www.jimmunol.org/content/156/10/3919.full
SO  - J. Immunol.1996 May 15; 156
AB  - BALB/c mice are often considered "low responders" or even "nonresponders" with regard to cytolytic CD8+ T lymphocytes and SV40 large T Ag (TAg). Large TAg and fragments thereof were produced by recombinant technology and injected into BALB/c mice that were subsequently challenged by i.p. injection of syngeneic TAg-expressing mKSA tumor cells. Two portions of the TAg were found to induce protective immunity, one stretching from amino acid residues 1-272 and the other from amino acid residues 683-708. In mice thus protected, the spleens were virtually free of cytotoxic T cells but CD8+ T lymphocytes obtained from the peritoneal cavity during rejection of the mKSA cells were directly lytic for TAg-expressing target cells. Depleting immune mice of CD4+ or CD8+ T lymphocytes by treatment with mAb abolished their ability to resist tumor development. We conclude that immunity against SV40 TAg-expressing tumor cells in BALB/c mice is dependent on both CD4+ and CD8+ T lymphocytes.