PT  - JOURNAL ARTICLE
AU  - Fellah, J S
AU  - Kerfourn, F
AU  - Charlemagne, J
TI  - Evolution of T cell receptor genes. Extensive diversity of V beta families in the Mexican axolotl.
DP  - 1994 Nov 15
TA  - The Journal of Immunology
PG  - 4539--4545
VI  - 153
IP  - 10
4099  - http://www.jimmunol.org/content/153/10/4539.short
4100  - http://www.jimmunol.org/content/153/10/4539.full
SO  - J. Immunol.1994 Nov 15; 153
AB  - We have cloned 36 different rearranged variable regions (V beta) genes encoding the beta-chain of the T cell receptor in an amphibian species, Ambystoma mexicanum (the Mexican axolotl). Eleven different V beta segments were identified, which can be classified into 9 families on the basis of a minimum of 75% nucleotide identity. All the cloned V beta segments have the canonical features of known mammalian and avian V beta, including conserved residues Cys23, Trp34, Arg69, Tyr90, and Cys92. There seems to be a greater genetic distance between the axolotl V beta families than between the different V beta families of any mammalian species examined to date: most of the axolotl V beta s have fewer than 35% identical nucleotides and the less related families (V beta 4 and V beta 8) have no more than 23.2% identity (13.5% at the amino acid level). Despite their great mutual divergence, several axolotl V beta are sequence-related to some mammalian V beta genes, like the human V beta 13 and V beta 20 segments and their murine V beta 8 and V beta 14 homologues. However, the axolotl V beta 8 and V beta 9 families are not significantly related to any other V beta sequence at the nucleotide level and show limited amino acid similarity to mammalian V alpha, V kappa III, or VH sequences. The detection of nine V beta families among 35 randomly cloned V beta segments suggests that the V beta gene repertoire in the axolotl is probably larger than presently estimated.