RT Journal Article
SR Electronic
T1 Aged T cells are hyporesponsive to costimulation mediated by CD28.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 3740
OP 3747
VO 152
IS 8
A1 Engwerda, C R
A1 Handwerger, B S
A1 Fox, B S
YR 1994
UL http://www.jimmunol.org/content/152/8/3740.abstract
AB The ability of T cells to proliferate in response to a number of stimuli is impaired in healthy, aged individuals. T cells from young (2 to 4 mo) and aged (20 to 26 mo) mice were stimulated with immobilized anti-CD3 epsilon-chain mAb and soluble anti-CD28 mAb. T cells from young and aged mice proliferated comparably in response to anti-CD3 epsilon mAb alone. However, aged T cells were significantly less responsive to costimulation by anti-CD28 mAb. This decreased response of aged T cells was seen at all dosages tested of anti-CD3 epsilon and anti-CD28 mAbs and in the presence and absence of APC. Similar results were observed when costimulation was provided by B7-transfected L cell fibroblasts. T cells from young and aged mice had comparable expression of CD28 by flow cytometry, both before and after stimulation. The defect in response to CD28 was seen both in CD4+ and CD8+ T cells and in CD44lo (naive) and CD44hi (memory) T cells. The impaired response to costimulation mediated by CD28 on T cells from aged mice may be an important factor in reduced T cell responses associated with aging.