RT Journal Article
SR Electronic
T1 Transformation of T lymphocytes by the v-fos oncogene.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 4355
OP 4364
VO 145
IS 12
A1 Valge-Archer, V E
A1 de Villiers, J
A1 Sinskey, A J
A1 Rao, A
YR 1990
UL http://www.jimmunol.org/content/145/12/4355.abstract
AB Activation of T lymphocytes through the T cell antigen receptor has been shown to stimulate a rapid and transient accumulation of c-fos mRNA and protein. Transfection of a normal murine T lymphocyte clone with the FBJ-v-fos oncogene resulted in generation of a cell line that was morphologically transformed, had lost the requirement for IL-2 for proliferation, and was tumorigenic in adult syngeneic mice; however, the transformed cells retained the ability to proliferate in response to IL-2. The transformed cells did not show constitutive expression of IL-2 or c-fos mRNA, although the promoter regions of both IL-2 and c-fos genes contain AP-1 sites that are expected to be targets for binding of Fos/Jun complexes. In contrast, the transformed T cells showed increased constitutive expression of IL-2R alpha and c-myc mRNA; these genes may represent cellular targets for transformation by v-fos and physiologic activation by c-fos. We discuss the possibility that these transformed cells behave as cells partially activated through the TCR, and that transformation occurs through a mechanism independent of IL-2.