RT Journal Article
SR Electronic
T1 Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 2736
OP 2742
VO 142
IS 8
A1 Kubo, R T
A1 Born, W
A1 Kappler, J W
A1 Marrack, P
A1 Pigeon, M
YR 1989
UL http://www.jimmunol.org/content/142/8/2736.abstract
AB Research on the specificities, functions, and maturation of T cells would be greatly aided by a collection of monoclonal antibodies which distinguishes different types of TCR. With this end in mind hamsters were immunized and tested for production of pan-reactive anti-mouse alpha beta TCR antibodies. In this report we describe the properties and uses of a mAb, H57-597, produced from one of these animals. The mAb reacts with surface receptors on all alpha beta TCR-bearing cells and does not react with receptors on gamma delta+ T cells. In an immobilized form, this antibody can directly activate T cells bearing alpha beta TCR. It can be used in immunoprecipitation reactions to precipitate receptor from the appropriate cell types. In combination with anti-CD3, the antibody can be used in cytofluorographic analyses to measure numbers of CD3+, alpha beta+, and CD3+, gamma delta+ cells in the thymus and periphery.