PT  - JOURNAL ARTICLE
AU  - Kubo, R T
AU  - Born, W
AU  - Kappler, J W
AU  - Marrack, P
AU  - Pigeon, M
TI  - Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
DP  - 1989 Apr 15
TA  - The Journal of Immunology
PG  - 2736--2742
VI  - 142
IP  - 8
4099  - http://www.jimmunol.org/content/142/8/2736.short
4100  - http://www.jimmunol.org/content/142/8/2736.full
SO  - J. Immunol.1989 Apr 15; 142
AB  - Research on the specificities, functions, and maturation of T cells would be greatly aided by a collection of monoclonal antibodies which distinguishes different types of TCR. With this end in mind hamsters were immunized and tested for production of pan-reactive anti-mouse alpha beta TCR antibodies. In this report we describe the properties and uses of a mAb, H57-597, produced from one of these animals. The mAb reacts with surface receptors on all alpha beta TCR-bearing cells and does not react with receptors on gamma delta+ T cells. In an immobilized form, this antibody can directly activate T cells bearing alpha beta TCR. It can be used in immunoprecipitation reactions to precipitate receptor from the appropriate cell types. In combination with anti-CD3, the antibody can be used in cytofluorographic analyses to measure numbers of CD3+, alpha beta+, and CD3+, gamma delta+ cells in the thymus and periphery.