PT - JOURNAL ARTICLE AU - Clark-Lewis, I AU - Lopez, A F AU - To, L B AU - Vadas, M A AU - Schrader, J W AU - Hood, L E AU - Kent, S B TI - Structure-function studies of human granulocyte-macrophage colony-stimulating factor. Identification of residues required for activity. DP - 1988 Aug 01 TA - The Journal of Immunology PG - 881--889 VI - 141 IP - 3 4099 - http://www.jimmunol.org/content/141/3/881.short 4100 - http://www.jimmunol.org/content/141/3/881.full SO - J. Immunol.1988 Aug 01; 141 AB - Human granulocyte-macrophage colony stimulating factor (hGM CSF), a protein containing 127 amino acids, was chemically synthesized by using automated stepwise solid-phase methods. The unpurified synthetic hGM-CSF had the same range of actions on hemopoietic cells as the purified recombinant protein. The structural requirements for the activities of synthetic hGM-CSF were examined by the design and synthesis of fragments and analogs. The synthetic fragment, hGM-CSF (54-127), containing all four of the cysteine residues found in the intact protein, lacked detectable activity. Assays of fragments shortened at the N terminus showed that the residues 1-13 were not required for activity, but that the integrity of residues 14-25, particularly residues 16, 17, and 18, was critical for biologic activity. The 14-25 region is predicted to form the first alpha-helix in hGM-CSF. Synthetic peptides within the N-terminal 53 residue region lacked detectable activity. The synthetic analog hGM-CSF (1-121), which lacks the C-terminal 6 residues, had similar activity to hGM-CSF (1-127) indicating that residues 122-127 are not required for activity. An analog, [Ala88] hGM-CSF (14-96), which lacks the hydrophobic C-terminal region and 2 cysteine residues, had low but readily detectable activity suggesting that residues 14-96 are sufficient for detectable synthetic hGM-CSF activity, although the presence of residues 97-121 are required for full activity. No dissociation of the multiple biological activities of hGM-CSF was detected.