PT  - JOURNAL ARTICLE
AU  - Cunningham-Rundles, C
AU  - Feng, Z K
TI  - Analysis of a common inheritable idiotype in IgA-deficient sera using monoclonal antibodies.
DP  - 1988 Jun 01
TA  - The Journal of Immunology
PG  - 3880--3886
VI  - 140
IP  - 11
4099  - http://www.jimmunol.org/content/140/11/3880.short
4100  - http://www.jimmunol.org/content/140/11/3880.full
SO  - J. Immunol.1988 Jun 01; 140
AB  - In these studies we describe the production of three mAb raised to an idiotype on an IgG anticasein antibody isolated from the serum of one IgA-deficient blood donor. These are IgM kappa and block the binding of casein Ag to anticasein antibody. Sera of unrelated IgA-deficient donors were tested for the presence of the idiotype; 15 of 56 IgA-deficient sera (25%) contain the anticasein idiotype, whereas 1 of 45 normal sera was positive. Anticasein antibodies as a whole were predominantly of the IgG1 and IgG3 subclass; idiotype-positive anticaseins are predominantly of the IgG1 subclass. For IgA-deficient donors, the relative amount of idiotype-positive anticasein antibody was correlated with the level of anticasein present in the serum. Studies were done to investigate the potential inheritance of the idiotype in families; in three of four families the idiotype was inherited in an apparent autosomal dominant pattern. Our data show that a common cross-reactive idiotype can be detected in the sera of IgA-deficient individuals and their family members. This suggests that V region markers may be conserved in this humoral immunodeficiency disease.