RT Journal Article
SR Electronic
T1 Expression of immunoglobulin lambda light chain by the promyelocytic cell line HL-60.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 3978
OP 3982
VO 137
IS 12
A1 Stavnezer, J
A1 Al-Katib, A
A1 Koziner, B
YR 1986
UL http://www.jimmunol.org/content/137/12/3978.abstract
AB The HL-60 cell line, established from a patient with acute promyelocytic leukemia, can be induced to undergo differentiation along the granulocyte or monocyte/macrophage line, depending on the particular inducer that is used. In this communication we provide evidence that HL-60 cells also have B lymphoid characteristics because by flow cytometry and clonal excess calculations, these cells are found to express immunoglobulin (Ig) lambda light chains on their surface. Furthermore, HL-60 cells contain poly(A)+ RNA that hybridizes with a DNA fragment encoding the constant region of Ig lambda chains and comigrates with lambda mRNA on RNA blots. Treatment of HL-60 cells with a phorbol ester that induces monocyte/macrophage differentiation resulted in the loss of surface Ig lambda chains and lambda RNA.