PT  - JOURNAL ARTICLE
AU  - Jeske, D
AU  - Milner, E C
AU  - Leo, O
AU  - Moser, M
AU  - Marvel, J
AU  - Urbain, J
AU  - Capra, J D
TI  - Molecular mapping of idiotopes of anti-arsonate antibodies.
DP  - 1986 Apr 01
TA  - The Journal of Immunology
PG  - 2568--2574
VI  - 136
IP  - 7
4099  - http://www.jimmunol.org/content/136/7/2568.short
4100  - http://www.jimmunol.org/content/136/7/2568.full
SO  - J. Immunol.1986 Apr 01; 136
AB  - As part of understanding molecular function in structural terms, we have been attempting to map the idiotypic topography of specific anti-arsonate (Ars) antibodies. A panel of anti-Ars hybridomas of which the complete primary sequences are known were used. These molecules show a varied reactivity profile with a panel of monoclonal anti-idiotypic antibodies. By judicious chain recombination experiments and chemical modifications that altered this reactivity profile, we were able to identify particular amino acid residues or discreet regions of anti-Ars antibodies as having crucial roles in the expression of idiotypic determinants. Idiotopes were mapped to the heavy chain second hypervariable region and D segment, and to the light chain first and third hypervariable regions.