PT  - JOURNAL ARTICLE
AU  - Gowen, M
AU  - Mundy, G R
TI  - Actions of recombinant interleukin 1, interleukin 2, and interferon-gamma on bone resorption in vitro.
DP  - 1986 Apr 01
TA  - The Journal of Immunology
PG  - 2478--2482
VI  - 136
IP  - 7
4099  - http://www.jimmunol.org/content/136/7/2478.short
4100  - http://www.jimmunol.org/content/136/7/2478.full
SO  - J. Immunol.1986 Apr 01; 136
AB  - Human peripheral blood cells, when cultured in vitro, release bone-resorbing factors, which have been called osteoclast-activating factors (OAF) but remain unidentified. We showed previously that a monocyte product, similar to interleukin 1 (IL 1), is a powerful stimulator of bone resorption in vitro. However, the possibility remained that other immune cell products may contribute to OAF activity. We have therefore tested three recombinant cytokines; IL 1, interleukin 2 (IL 2), and interferon-gamma (IFN-gamma) for their activity in a neonatal mouse bone resorption assay. We report here that purified recombinant murine IL 1 is a potent and powerful stimulator of bone resorption in vitro, active over a concentration range of 0.14 to 33 U/ml (1.3 X 10(-12) to 3.1 X 10(-10) M). IL 1-stimulated bone resorption was unaffected by cyclooxygenase inhibition but was inhibited by calcitonin and IFN-gamma. IL 2 had no effect on bone resorption.