RT Journal Article
SR Electronic
T1 Amphotericin B inhibits the serum-induced expression of tissue transglutaminase in murine peritoneal macrophages.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 4206
OP 4212
VO 136
IS 11
A1 Mehta, K
A1 Claringbold, P
A1 Lopez-Berestein, G
YR 1986
UL http://www.jimmunol.org/content/136/11/4206.abstract
AB Culture of mouse resident peritoneal macrophages (PM) in serum-containing medium causes a rapid and marked induction of the enzyme tissue transglutaminase (tissue TGase). Coculture of PM with amphotericin B (AmpB) inhibited the serum-induced expression and accumulation of tissue TGase. The AmpB-mediated inhibition of tissue TGase was specific and was due to inhibition of enzyme synthesis. The serum-dependent induction of tissue TGase was inhibited in a dose-dependent fashion, and a complete inhibition was observed at 1.5 microgram/ml dose of AmpB. The inhibition was reversible; however, the time of recovery depended on the dose and time of exposure of the cells to AmpB. The present studies suggest that AmpB-mediated inhibition of tissue TGase is due to inhibition of the uptake of serum retinoids by PM.