RT Journal Article
SR Electronic
T1 Studies on natural killer (NK) cells. II. Serologic evidence for heterogeneity of murine NK cells.
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 1864
OP 1868
VO 127
IS 5
A1 Burton, R C
A1 Bartlett, S P
A1 Kumar, V
A1 Winn, H J
YR 1981
UL http://www.jimmunol.org/content/127/5/1864.abstract
AB NK cells are divided into 2 subclasses on the basis of cell surface antigens: NKA cells, which preferentially lyse lymphoma cells and which are readily destroyed by treatment with anti-H-2, rabbit anti-mouse, or anti-NK specific antibodies in the presence of C; and NKB cells, which preferentially lyse nonlymphoma tumor cells and which are resistant to anti-NK specific antibodies, partially sensitive to anti-H-2 specific antibodies, and only fully sensitive to rabbit anti-mouse serum and C. Thus, suspensions of spleen cells that were treated with anti-NK-1.2 antibodies and C lost virtually all lytic activity for 6 lymphoma cell lines, whereas lytic activity for 2 nonlymphoma cell lines remained largely intact. It must be noted, however, that unlike NK-1.2 expression, target cell preference is not an absolute indicator of NK cell subclass. Cytotoxic T cells were readily distinguished from NKA and NKB cells on the basis of their sensitivity to monoclonal anti-Thy-1.2 antibodies and resistance to anti-NK-1.2 antiserum and C.