PT  - JOURNAL ARTICLE
AU  - Emerson, S G
AU  - Cone, R E
TI  - I-Kk and H-2Kk antigens are shed as supramolecular particles in association with membrane lipids.
DP  - 1981 Aug 01
TA  - The Journal of Immunology
PG  - 482--486
VI  - 127
IP  - 2
4099  - http://www.jimmunol.org/content/127/2/482.short
4100  - http://www.jimmunol.org/content/127/2/482.full
SO  - J. Immunol.1981 Aug 01; 127
AB  - The form of 125I-labeled membrane alloantigens (I-Ak and H-2Kk) and immunoglobulin M shed by murine splenic lymphocytes labeled by lactoperoxidase-catalyzed radioiodination was determined by gel chromatography and ultracentrifugation. I-Ak and H-2Kk antigens are shed as particles ranging in size from 3 X 10(5) to 2 X 10(6), whereas membrane IgM is shed as an individual unit consonant with its native size of 180,000. The effects of detergents on the size of shed I-Ak and H-2Kk and the coprecipitation of (3H) lipids with these proteins suggest that I-Ak and H-2Kk are shed in association with local membrane lipids as small membrane-derived vesicles, whereas membrane IgM is shed without detectable associated lipid.