RT Journal Article
SR Electronic
T1 Identification and Separation of Thy-1 Positive Mouse Spleen Cells Active in Natural Cytotoxicity and Antibody-Dependent Cell-Mediated Cytotoxicity
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 2851
OP 2860
VO 123
IS 6
A1 Mattes, M. Jules
A1 Sharrow, Susan O.
A1 Herberman, Ronald B.
A1 Holden, Howard T.
YR 1979
UL http://www.jimmunol.org/content/123/6/2851.abstract
AB The expression of the Thy-1 antigen on mouse spleen cells responsible for NK activity and ADCC was investigated by using a monoclonal IgM anti-Thy-1.2 antibody. Both C-mediated cytotoxicity and the fluorescence-activated cell sorter were used to fractionate cells. The effector cells were found to be heterogeneous in their expression of Thy-1. Effector cells from nude BALB/c mice were predominantly Thy-1 positive; some of the NK cells in CBA spleens appeared to be Thy-1 positive, but at least one-third of the lytic activity was due to Thy-1 negative cells. The effects of treatments on NK cytotoxicity and ADCC were very similar, supporting the hypothesis that the same cells mediate both activities.