PT  - JOURNAL ARTICLE
AU  - Novogrodsky, Abraham
AU  - Lotan, Reuben
AU  - Ravid, Amiram
AU  - Sharon, Nathan
TI  - Peanut Agglutinin, A New Mitogen That Binds to Galactosyl Sites Exposed after Neuraminidase Treatment
DP  - 1975 Nov 01
TA  - The Journal of Immunology
PG  - 1243--1248
VI  - 115
IP  - 5
4099  - http://www.jimmunol.org/content/115/5/1243.short
4100  - http://www.jimmunol.org/content/115/5/1243.full
SO  - J. Immunol.1975 Nov 01; 115
AB  - Peanut agglutinin, purified by affinity chromatography, agglutinates lymphocytes from mouse, rat, guinea pig, and man only after their treatment with neuraminidase. However, it stimulates only neuraminidase-treated rat and human cells. A similar number of cell surface receptors for peanut agglutinin was found on neuraminidase-treated rat and mouse lymphocytes although the latter cells were not stimulated by the lection. Galactose specifically inhibited the agglutination and stimulation of lymphocytes by peanut agglutinin. Sequential treatment of lymphocytes with neuraminidase and β-galactosidase markedly reduced the response of the cells to stimulation by peanut agglutinin, soybean agglutinin, and galactose oxidase. It is suggested that the same galactosyl residue may be the target for the initial step in triggering lymphocytes by the above mentioned mitogens.