RT Journal Article
SR Electronic
T1 Lymphatic Cannulation for Lymph Sampling and Molecular Delivery
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 2339
OP 2350
DO 10.4049/jimmunol.1900375
VO 203
IS 8
A1 Zawieja, David C.
A1 Thangaswamy, Sangeetha
A1 Wang, Wei
A1 Furtado, Raquel
A1 Clement, Cristina C.
A1 Papadopoulos, Zachary
A1 Vigano, Marco
A1 Bridenbaugh, Eric A.
A1 Zolla, Lello
A1 Gashev, Anatoliy A.
A1 Kipnis, Jonathan
A1 Lauvau, Gregoire
A1 Santambrogio, Laura
YR 2019
UL http://www.jimmunol.org/content/203/8/2339.abstract
AB Lymph carries the “omic,” vesicular, and immune cell signature of the draining organs.Lymph analysis provides precise and “undiluted” biochemical and cellular information.A protocol for lymph collection from mouse and rat lymphatics is reported.Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the “omic” sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ’s biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ’s physiology/pathology, making it an ideal target for liquid biopsy.