PT  - JOURNAL ARTICLE
AU  - Leslie, Gerrie A.
AU  - Clem, L. W.
TI  - Chicken Antibodies to Group a Streptococcal Carbohydrate
DP  - 1973 Jan 01
TA  - The Journal of Immunology
PG  - 191--196
VI  - 110
IP  - 1
4099  - http://www.jimmunol.org/content/110/1/191.short
4100  - http://www.jimmunol.org/content/110/1/191.full
SO  - J. Immunol.1973 Jan 01; 110
AB  - Adult chickens were hyperimmunized with group A streptococcal vaccine in an attempt to elicit the formation of high levels of antibody of restricted heterogeneity. All birds produced precipitating antibody after 3 weeks of immunization. The highest concentration was ∼8.6 mg/ml. After 6 weeks of immunization the highest level was ∼13 mg/ml and even after 6 weeks most birds had both IgM and IgY. Approximately 10% of specifically purified antibody preparations contained some IgA. In each case this was associated with the macroglobulin fraction of Sephadex G-200. Of the 37 different antibody preparations examined only ∼5% (two IgM preparations) showed a clear restriction in L chain heterogeneity. These data indicate that like rabbits and sharks, chickens can produce high levels of antibody to a group A streptococcal carbohydrate with some antibody preparations showing restricted heterogeneity.