RT Journal Article
SR Electronic
T1 Antigen Recognition in the Islets Changes with Progression of Autoimmune Islet Infiltration
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 522
OP 530
DO 10.4049/jimmunol.1400626
VO 194
IS 2
A1 Lindsay, Robin S.
A1 Corbin, Kaitlin
A1 Mahne, Ashley
A1 Levitt, Bonnie E.
A1 Gebert, Matthew J.
A1 Wigton, Eric J.
A1 Bradley, Brenda J.
A1 Haskins, Kathryn
A1 Jacobelli, Jordan
A1 Tang, Qizhi
A1 Krummel, Matthew F.
A1 Friedman, Rachel S.
YR 2015
UL http://www.jimmunol.org/content/194/2/522.abstract
AB In type 1 diabetes, the pancreatic islets are an important site for therapeutic intervention because immune infiltration of the islets is well established at diagnosis. Therefore, understanding the events that underlie the continued progression of the autoimmune response and islet destruction is critical. Islet infiltration and destruction is an asynchronous process, making it important to analyze the disease process on a single islet basis. To understand how T cell stimulation evolves through the process of islet infiltration, we analyzed the dynamics of T cell movement and interactions within individual islets of spontaneously autoimmune NOD mice. Using both intravital and explanted two-photon islet imaging, we defined a correlation between increased islet infiltration and increased T cell motility. Early T cell arrest was Ag dependent and due, at least in part, to Ag recognition through sustained interactions with CD11c+ APCs. As islet infiltration progressed, T cell motility became Ag independent, with a loss of T cell arrest and sustained interactions with CD11c+ APCs. These studies suggest that the autoimmune T cell response in the islets may be temporarily dampened during the course of islet infiltration and disease progression.