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The Porcine and Chicken Innate DNA Sensing cGAS-STING-IRF Signaling Axes Exhibit Differential Species Specificity

Sen Jiang, Jia Luo, Youwen Zhang, Qi Cao, Yuening Wang, Nengwen Xia, Wanglong Zheng, Nanhua Chen, François Meurens, Huiguang Wu and Jianzhong Zhu
J Immunol July 1, 2022, ji2101212; DOI: https://doi.org/10.4049/jimmunol.2101212
Sen Jiang
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Jia Luo
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Youwen Zhang
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Qi Cao
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Yuening Wang
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Nengwen Xia
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Wanglong Zheng
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Nanhua Chen
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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François Meurens
¶Biologie, Épidémiologie et Analyse de Risque en santé animale, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Oniris, Nantes, France; and
‖Department of Veterinary Microbiology and Immunology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
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Huiguang Wu
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Jianzhong Zhu
*Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China;
†College of Veterinary Medicine, Yangzhou University, Yangzhou, China;
‡Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China;
§Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;
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Key Points

  • Porcine and chicken STING but not cGAS exhibit species disparity.

  • The species specificity of STING matches up to downstream IRF3/7.

  • IRF3 works with only porcine STING, whereas IRF7 works with both porcine and chicken STING.

Abstract

The innate immune DNA sensing cyclic GMP-AMP synthase (cGAS)–stimulator of IFN genes (STING) signaling pathway plays a key role in host antiviral function. Although the cGAS-STING pathway has been extensively studied, the cGAS-STING signaling in livestock and poultry is not well understood, and whether the species specificity exists is still unknown. In this study, we found that porcine and chicken STING, but not cGAS, exhibit species differences in regulation of IFN; that is, porcine (p)STING mediates good induction of IFN in mammalian cells and low IFN induction in chicken DF-1 cells; on the contrary, chicken (ch)STING mediates IFN induction only in chicken cells but not in mammalian cells. Furthermore, it was found that the motifs pLxIS of pSTING and pLxVS of chSTING are responsible for the species disparity, with the IFN activity of pSTING and chSTING exchanged by swapping the two pLxI/VS motifs. The pLxI/VS motifs mediated the interactions of various STING with downstream IFN regulatory factors (IRFs), reflecting the species-specific pIRF3 and chIRF7. Next, the STING, IRFs, and STING-IRFs were reconstituted in porcine and chicken macrophages that were genetically knocked out for STING and/or IRFs by the CRISPR-Cas9 approach. The results showed that pSTING plus pIRF3 or chIRF7 are able to induce IFN; however, chSTING plus chIRF7 but not pIRF3 are able to induce IFN, suggesting that pIRF3 is specific and stringent, which underlies the inability of chSTING to induce IFN in mammalian cells. In summary, our findings reveal the differential species specificity in the cGAS-STING pathway and the underlying mechanisms, thus providing valuable insights on the cGAS-STING-IRF signaling axis for comparative immunology.

Footnotes

  • This work was supported by the Innovative Research Group Project of the National Natural Science Foundation of China (Grants 32172867 and 31872450) and by a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. S.J. was supported by the Yangzhou University Postgraduate Research and Practice Innovation Program of Jiangsu Province (Grant KYCX21_3272).

  • The online version of this article contains supplemental material.

  • Received January 6, 2022.
  • Accepted May 18, 2022.
  • Copyright © 2022 by The American Association of Immunologists, Inc.

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The Journal of Immunology: 209 (3)
The Journal of Immunology
Vol. 209, Issue 3
1 Aug 2022
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The Porcine and Chicken Innate DNA Sensing cGAS-STING-IRF Signaling Axes Exhibit Differential Species Specificity
Sen Jiang, Jia Luo, Youwen Zhang, Qi Cao, Yuening Wang, Nengwen Xia, Wanglong Zheng, Nanhua Chen, François Meurens, Huiguang Wu, Jianzhong Zhu
The Journal of Immunology July 1, 2022, ji2101212; DOI: 10.4049/jimmunol.2101212

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The Porcine and Chicken Innate DNA Sensing cGAS-STING-IRF Signaling Axes Exhibit Differential Species Specificity
Sen Jiang, Jia Luo, Youwen Zhang, Qi Cao, Yuening Wang, Nengwen Xia, Wanglong Zheng, Nanhua Chen, François Meurens, Huiguang Wu, Jianzhong Zhu
The Journal of Immunology July 1, 2022, ji2101212; DOI: 10.4049/jimmunol.2101212
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