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Physicochemical Properties of Reaginic Antibody

V. Correlation of Reaginic Activity with γE-Globulin Antibody

Kimishige Ishizaka, Teruko Ishizaka and Margaret M. Hornbrook
J Immunol December 1, 1966, 97 (6) 840-853;
Kimishige Ishizaka
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Teruko Ishizaka
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Margaret M. Hornbrook
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Summary

The skin-sensitizing activity in the sera of nine atopic patients, who are sensitive to ragweed pollen, and in a reagin-rich fraction of another patient's serum was precipitated by anti-γE-globulin antiserum which did not contain any detectable antibody against γG-, γA-, γM- or γD-globulins. Absorption of the skin-sensitizing antibody was accompanied by the loss of γE antibody in the supernatants. The skin-sensitizing activity in the patients' sera, determined by P-K reactions with antigen E, paralleled radioactivity of the γE band which was observed by radioimmunodiffusion with the radioactively-labeled antigen, whereas no correlation was observed between the skin-sensitizing activity and either γG or γA antibodies. When one of the patients' sera was fractionated by chromatography on a DEAE cellulose column, gel filtration, sucrose density gradient ultracentrifugation and by zone electrophoresis on agarose gel, distributions of reaginic activity paralleled those of γE antibody. These findings strongly support the hypothesis that reaginic antibodies in ragweed sensitive patients' sera are associated with γE-globulin. Light chains of immunoglobulins were detected in γE-globulin, indicating that γE-globulin is a new immunoglobulin.

Footnotes

  • ↵1 This work was supported mainly by research grants GB-4646 from the National Science Foundation and in part by Grant HE-05997 from the National Institutes of Health and United States Navy Contract Nonr-3656(12).

  • Received July 18, 1966.
  • Copyright © 1966 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 97, Issue 6
1 Dec 1966
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Physicochemical Properties of Reaginic Antibody
Kimishige Ishizaka, Teruko Ishizaka, Margaret M. Hornbrook
The Journal of Immunology December 1, 1966, 97 (6) 840-853;

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Physicochemical Properties of Reaginic Antibody
Kimishige Ishizaka, Teruko Ishizaka, Margaret M. Hornbrook
The Journal of Immunology December 1, 1966, 97 (6) 840-853;
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Print ISSN 0022-1767        Online ISSN 1550-6606