Summary
A simplified method is described for coupling proteins to red cells using the water-soluble carbodiimide, 1 - ethyl - 3 - (3 - dimethylaminopropyl) carbodiimide hydrochloride. The procedure is simpler than BDB coupling and the sensitized cells are more stable than those in the BDB system. The data indicated a marked similarity in the specificity and sensitivity of ECDI-and BDB-hemagglutination.
- Received July 5, 1966.
- Copyright © 1966 by The American Association of Immunologists, Inc.
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In this issue
The Use of a Water-Soluble Carbodiimide as a Coupling Reagent in the Passive Hemagglutination Test
