Summary
Purified anti-dinitrophenyl group antibodies from pooled antisera or antiserum of a single rabbit were fractionated by means of homologous and cross-reacting immunoadsorbents. Multiple fractions were obtained from all immunoadsorbents by means of stepwise hapten elution with dinitrophenol and when the homologous immunoadsorbent, 2,4-dinitrophenylaminoethyl cellulose, or the extensively altered immunoadsorbent, nitrobenzyl cellulose, were used only a few fractions or a single fraction were obtained. The most effective immunoadsorbent was found to be 2,4,6-trinitrophenylaminoethyl cellulose, which yielded eight major fractions of anti-DNP from the serum of a single rabbit. The chromatographic behavior appears primarily to be determined by differences of the binding ratio of various antibody fractions for the immunoadsorbent and the eluting hapten.
Footnotes
-
↵1 Supported by Grant No. AI 03047 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. A preliminary report was presented at the 150th National Meeting of the American Chemical Society, September 13 to 17, 1965.
- Received June 27, 1966.
- Copyright © 1966 by The American Association of Immunologists, Inc.
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
Log in using your username and password
In this issue
