Summary
Sera from calves inoculated with a cytopathogenic bovine viral diarrhea (BVD) virus, a noncytopathogenic BVD virus, or with a soluble antigen (SA) from a BVD virus were fractionated into heavy (19 S) and light (7 S) components of γ-globulins by sucrose gradient ultracentrifugation. These antiserum fractions were tested for precipitating antibodies, for fluorescing antibodies, and for their capacities to neutralize BVD virus in cell cultures. Most 19 S fractions elicited lines of precipitation with a SA of BVD virus, but only the 7 S fraction produced from injections of SA into a calf precipitated, and only weakly, with the BVD-SA in Ouchterlony plates. All fractions, except a 19 S, combined with viral antigen produced in swine kidney cell line (PK-15) cultures in an indirect fluorescent antibody test.
The 19 S fraction predominated in the bovine sera studied and persisted for as long as 20 weeks. Restimulation of animals with antigen did not produce a secondary effect and the “early-19 S,” “late-7 S” responses were not present in this immunologic system.
The results of these studies are discussed in relation to studies of heterogeneity of other antigen-antibody systems, especially those dealing with viral systems.
- Received September 24, 1965.
- Copyright © 1966 by The American Association of Immunologists, Inc.
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