Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Weight Estimates of Rabbit Anti-Human Serum Albumin Based on Antigen-Binding Capacity

James J. Mulligan Jr., Abraham G. Osler and Eugene Rodriguez
J Immunol February 1, 1966, 96 (2) 324-333;
James J. Mulligan Jr.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abraham G. Osler
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eugene Rodriguez
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Summary

A quantitative technique is described for weight estimations of anti-human serum albumin (HSA) based on the primary union of the specific reactants rather than on the formation of insoluble aggregates. Trace-labeled I125·HSA is added, in quantities adequate to supply a great excess of antigen, to a series of tubes containing a constant volume of rabbit anti-human serum albumin. The globulins are precipitated and washed with half saturated ammonium sulfate, and assayed for I125·HSA. These values are converted to weight units of HSA, after appropriate correction, and are in turn used to calculate the absolute amount of antibody in the serum.

The results obtained in these assays are relatively insensitive to such variables as pH, duration of antigen-antibody incubation, and the presence of ethylenediaminetetraacetic acid.

A recovery assay with sheep anti-rabbit γ-globulin serum gave results similar to those obtained by mABC and by quantitative precipitin analyses.

The specificity of the assay system has been investigated with rabbit antisera to egg albumin, bovine serum albumin, human γ-globulin, and pneumococcus polysaccharide Type VI.

Footnotes

  • ↵1 Submitted to the School of Hygiene and Public Health, The Johns Hopkins University, in conformity with the requirements for the degree of Master of Science.

    Support for this investigation has been available, in part, from the National Science Foundation, Grant No. G-6205; The American Cancer Society, Inc., Grant No. T-257; The National Institute of Allergy and Infectious Diseases of the United States Public Health Service, Grant No. AI-03151; and from the Office of The Surgeon General, Department of the Army, under the auspices of the Commission on Immunization of the Armed Forces Epidemiological Board, Contract No. DA-49-193-MD-2468.

  • Received October 13, 1965.
  • Copyright © 1966 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
PreviousNext
Back to top

In this issue

The Journal of Immunology
Vol. 96, Issue 2
1 Feb 1966
  • Table of Contents
  • Table of Contents (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Weight Estimates of Rabbit Anti-Human Serum Albumin Based on Antigen-Binding Capacity
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Weight Estimates of Rabbit Anti-Human Serum Albumin Based on Antigen-Binding Capacity
James J. Mulligan, Abraham G. Osler, Eugene Rodriguez
The Journal of Immunology February 1, 1966, 96 (2) 324-333;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Weight Estimates of Rabbit Anti-Human Serum Albumin Based on Antigen-Binding Capacity
James J. Mulligan, Abraham G. Osler, Eugene Rodriguez
The Journal of Immunology February 1, 1966, 96 (2) 324-333;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606