Summary
A fluorometric method has been adapted to measure the relative concentrations of Groups A and A-variant antibodies in streptococcal Group A rabbit antisera. In this method FITC-labeled antibodies are adsorbed onto streptococcal cell walls and, after subsequent elution, measured in a fluorometer.
Inhibition of the reactions between Group A conjugate and homologous cell walls by Group A carbohydrate, and between Group A-variant conjugate and homologous cell walls by Group A-variant carbohydrate, confirmed the immunologic specificity of the two antibodies.
Footnotes
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↵1 This study was supported in part by National Institutes of Health Research Grant 08027, National Heart Institute; National Institutes of Health Research Grant 06154, National Institute of Allergy and Infectious Diseases, and National Institutes of Health Training Grant 5T1 GM 807, National Institutes of General Medical Science.
It was conducted in part under the sponsorship of the Commission on Streptococcal and Staphylococcal Diseases, Armed Forces Epidemiological Board, and was supported by the Offices of the Surgeon General, Department of the Army, Washington, D. C.
- Received July 19, 1965.
- Copyright © 1966 by The American Association of Immunologists, Inc.
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