Summary
A significant linear relationship was found to exist with both albumin (P < 0.05) and γG-globulin (P < 0.001) when mobility (µ) is plotted against the log of antigen concentration. Statistical analysis showed a significant difference (P < 0.025) between the µ value means of γG-globulin for the range of concentrations studied (0.168 to 5.38 mg N/ml); however, no difference (P >0.05) was detected between the value means for albumin (0.115 to 7.36 mg N/ml).
The only nonreacting component showing a significant effect on µ or p values (diffusivity ratios) was the effect of α1-globulin on the p values of the albumin system. By the separate addition of different concentrations of α1-globulin (0.120 to 3.85 mg N/ml final concentration) to a constant concentration of albumin (0.459 mg N/ml final concentration) the p value means for albumin plus α1-globulin were significantly higher than the p value means for the albumin control. The p value means for albumin plus α1-globulin were also found to be linearly related (P < 0.05) to the log of α1-globulin concentration. The significance of these data is discussed in reference to quantitative determinations by immunoelectrophoretic analysis. No significant effect on the µ or p value of γG-globulin was observed when β2-globulin was added to the γG-globulin-anti-γG-globulin system.
Footnotes
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↵1 The views expressed herein are those of the author and do not necessarily reflect the views of the United States Air Force or the Department of Defense.
- Received February 15, 1965.
- Copyright © 1965 by The American Association of Immunologists, Inc.
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