Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
      • Neuroimmunology: To Sense and Protect
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Biologic Characteristics of a Continuous Kidney Cell Line Derived from the African Green Monkey

Hope E. Hopps, Barbara C. Bernheim, Ananda Nisalak, Joe Hin Tjio and Joseph E. Smadel
J Immunol September 1, 1963, 91 (3) 416-424;
Hope E. Hopps
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Barbara C. Bernheim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ananda Nisalak
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joe Hin Tjio
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joseph E. Smadel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Discussion and Summary

A continuous cell line derived from kidney tissue of the African green monkey, Cercopithecus aethiops and designated BS-C-1, has been maintained through 142 subcultures. Cultures of the BS-C-1 line support the growth of simian virus 40 to the same extent as do primary Cercopithecus kidney cell cultures; moreover, the pathognomonic vacuolating changes induced by this virus are as apparent in the continuous cell line as in the primary cell cultures. The BS-C-1 cell cultures are suitable for propagating several viruses, including polio, measles, Rift Valley fever, respiratory syncytial, Coxsackie A9, O'Malley's A-1 agent, and simian agents 1, 4 and 5. In contrast, several strains of influenza A and B viruses and of adenoviruses 3, 4 and 7 as well as simian virus 2 failed to multiply in BS-C-1 cells.

No change in the susceptibility of the BS-C-1 cultures of SV40 virus was detected throughout 142 passages of the cell line. On the other hand, during this period of culture the cell line changed its chromosomal characteristics. Thus, the pattern remained diploid, i.e., 60 chromosomes, beyond the 20th subculture, and became subdiploid by the 41st passage after which it continued to change its characteristics with a majority of the cells in the 52nd passage having 59 chromosomes and the remainder 58; most of the cells had a dicentric chromosome. By the 111th passage, most of the cells approached the hypotetraploid state with 83% of the cells having 114 to 117 chromosomes. To our knowledge, this represents the first instance in which continuous observations have been made on the susceptibility of a tissue culture cell system to a given virus during a period when its cells changed from the diploid to the polyploid state. In this particular system the changes in chromosomal pattern were not associated with change in susceptibility to the virus. The BS-C-1 line is apparently free of extraneous microbial agents; seed cultures can be maintained in the frozen state; it grows rapidly and is not fastidious in its nutritional requirements.

The BS-C-1 continuous cell line provides the virologist with another tool for diagnostic and research work and perhaps for the large scale cultivation of viral agents for vaccines. As regards the last mentioned possibility the employment of continuous cell lines has recently received a cautious endorsement (22) for use in carefully planned, progressively expanding studies.

  • Received April 11, 1963.
  • Copyright, 1963, by The Williams & Wilkins Company
  • Copyright © 1963 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
PreviousNext
Back to top

In this issue

The Journal of Immunology
Vol. 91, Issue 3
1 Sep 1963
  • Table of Contents
  • Table of Contents (PDF)
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Biologic Characteristics of a Continuous Kidney Cell Line Derived from the African Green Monkey
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Biologic Characteristics of a Continuous Kidney Cell Line Derived from the African Green Monkey
Hope E. Hopps, Barbara C. Bernheim, Ananda Nisalak, Joe Hin Tjio, Joseph E. Smadel
The Journal of Immunology September 1, 1963, 91 (3) 416-424;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Biologic Characteristics of a Continuous Kidney Cell Line Derived from the African Green Monkey
Hope E. Hopps, Barbara C. Bernheim, Ananda Nisalak, Joe Hin Tjio, Joseph E. Smadel
The Journal of Immunology September 1, 1963, 91 (3) 416-424;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606