Summary and Conclusions
-
1. The allergenic and antigenic properties of pool C, a “purified” fraction of ragweed pollen, were investigated. As compared to whole ragweed pollen extract, pool C was found to be highly antigenic as measured by its ability to stimulate antibody production in rabbits, induce passive cutaneous anaphylaxis and provide indirect hemagglutination with both human and animal antisera.
-
2. Pool C is not immunochemically pure as evidenced by three or four lines produced by its homologous antisera in immunoelectrophoresis. However, many of the antigens in the whole ragweed extract have been eliminated.
-
3. Positive hemagglutination titers with pool C were found with the sera of 72% of untreated ragweed-sensitive individuals. This contrasted greatly with 20% positive reactions found with whole ragweed extract.
-
4. On a weight basis, pool C was 100 to 1000 times more skin-reactive than whole ragweed extract, both by direct and passive transfer tests.
-
5. In vitro and particularly in vivo neutralization tests indicated that some of the allergens in whole ragweed extract have been eliminated in the preparation of pool C.
Footnotes
-
↵1 Supported in part by Research Grant E-1303 and Training Grant 2E-51 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
- Received October 4, 1962.
- Copyright © 1963 by The American Association of Immunologists, Inc.
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$37.50
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.