Summary
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1. Experimental interpolation of slope K (diffusion (mm) vs √min) for SD columns of albumin homologous reactions resulted in the detection of concentration differences of 0.10 mg N2/ml (0.06% protein) of antigen and 0.010 mg N2/ml of antibody in 75% and 50% of the respective paired slope comparisons within specific antigen and antibody ranges.
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2. Comparison of the Kjeldahl concentrations with the same concentrations estimated from diffusion measurements for both antigen and antibody strengths in SD columns were made using results from regression of K on log of the appropriate reactant concentration. The data used were strictly selected for linearity and reproducibility. For 30 antigen derivations involving Fractions II and V homologous systems at three antibody levels, the mean deviation was 3.44%. The smallest deviation was -0.29% or 0.001 mg antigen N2 lower for the predicted as compared to the Kjeldahl concentrations.
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3. Estimated homologous antibody concentrations from rigidly selected data and curves (K regressed on the logarithm of the antibody concentrations), when compared with Kjeldahl values, showed an average deviation of 2.54% for 50 antibody derivations at five antigen levels for Fractions II and V in SD columns. The over-all mean difference (sign ignored) between Kjeldahl and diffusion—estimated values—was 0.007 mg antibody N2/ml.
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4. The limits of resolution for reactant concentration differences in SD column reactions at certain ranges are discussed in detail.
Footnotes
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↵1 The contents of this paper reflect the personal views of the author and are not to be construed as a statement of official Air Force policy.
- Received July 13, 1961.
- Copyright, 1962, by The Williams & Wilkins Company
- Copyright © 1962 by The American Association of Immunologists, Inc.
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