Quantitative Analyses by Diffusion Column Reactions

Summary

1. 1. Of 100 lines resulting from regression of diffusion (mm) onto √min for SD columns of fraction (II, V)-antifraction homologous reactions, 91% could be adequately expressed by a linear equation (y = K√min + a) when tested for good-of-fit (F-ratio test) over a wide range of antibody concentrations.

2. 2. At specific reactant concentrations, diffusion (mm) vs √min had 57% and 100% reproducibility for two series for Fractions II and V homologous SD reactions respectively (Table I).

3. 3. Analyses of standard curves with slope m, resulting from K regressed on the logarithm of wide ranges of antigen or antibody concentration, indicated that 19 out of 40 (47%) could be adequately expressed by a linear equation (K = m (log concentration + b)) (Table II).

4. 4. Considering only standard curves that gave adequate straight line fits and using stored SD columns, the resulting slopes m had good reproducibility (89%) even on only duplicate series of titrations (Table II).