Summary
The fluorescein-labelled antibody technique was employed in the localization of Forssman antigen in the tissues of guinea pig, cat, dog, mouse, and chicken. Antisera to sheep blood cells and to horse kidney were prepared in rabbits. The globulin fractions of the sera were labelled with fluorescein isocyanate. The specificity of the stain was established by several methods. The Forssman antigen was found in the form of droplets in the endothelium and adventitial connective tissues of the blood vessels in all organs of all species examined. Species differences in the distribution of Forssman antigen in other tissues are described.
Footnotes
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↵1 This work was supported by the American Heart Association.
- Received May 18, 1956.
- Copyright © 1956 by The American Association of Immunologists, Inc.
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