Summary
Dilution of chicken antisera prior to combination with the antigen causes a marked decrease in the antibody nitrogen precipitation. Application of the data to an adsorption equation permitted formulation of a “Desorption Equation” which was fairly successful in predicting antibody nitrogen values for diluted sera. This decrease in precipitation was also apparent with volume change of the undiluted reacting system. The explanation offered for the observed phenomenon was based on an adsorption mechanism along with multiplicity of antibody types believed to be present in antisera.
Footnotes
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↵1 Supported in part by the Research Committee of the University of Wisconsin Graduate School, from funds supplied by the Wisconsin Alumni Research Foundation.
- Received March 29, 1956.
- Copyright © 1956 by The American Association of Immunologists, Inc.
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