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Precipitin Production in Chickens

XIV. Effect of Dilution of Chicken Antisera on the Amount of Precipitation

Nazareth Gengozian and Harold R. Wolfe
J Immunol September 1, 1956, 77 (3) 172-180;
Nazareth Gengozian
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Harold R. Wolfe
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Summary

Dilution of chicken antisera prior to combination with the antigen causes a marked decrease in the antibody nitrogen precipitation. Application of the data to an adsorption equation permitted formulation of a “Desorption Equation” which was fairly successful in predicting antibody nitrogen values for diluted sera. This decrease in precipitation was also apparent with volume change of the undiluted reacting system. The explanation offered for the observed phenomenon was based on an adsorption mechanism along with multiplicity of antibody types believed to be present in antisera.

Footnotes

  • ↵1 Supported in part by the Research Committee of the University of Wisconsin Graduate School, from funds supplied by the Wisconsin Alumni Research Foundation.

  • Received March 29, 1956.
  • Copyright © 1956 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 77, Issue 3
1 Sep 1956
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Precipitin Production in Chickens
Nazareth Gengozian, Harold R. Wolfe
The Journal of Immunology September 1, 1956, 77 (3) 172-180;

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Precipitin Production in Chickens
Nazareth Gengozian, Harold R. Wolfe
The Journal of Immunology September 1, 1956, 77 (3) 172-180;
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Print ISSN 0022-1767        Online ISSN 1550-6606