Summary
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1. It has been shown that Ca++ as well as Mg++ are essential for immune hemolysis.
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2. Mg++ can be replaced with a fair degree of efficiency by Co++ or Ni++. The requirement for Ca++ could not be met by Co++, Mn++, Ni++, Mg++, Cd++, Sr++, Ba++, and Zn++.
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3. Evidence is presented showing that immune hemolysis takes place in at least two reaction steps: Step 1, involving C′ fixation requires Ca++; Step 2 is a reaction of an unknown nature, which requires Mg++.
- Received July 8, 1953.
- Copyright © 1953 by The American Association of Immunologists, Inc.
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