Summary
The role of Ca++ in C′ fixation has been studied by two methods: 1) With a system depleted of divalent cations by passage through a resin, Ca++, but not Mg++, caused a marked enhancement in C′ fixation; 2) With a system containing EDTA as an inhibitory chelating agent, Ca++, but not Mg++, Sr++, or Ba++, restores C′ fixation. Therefore Ca++ is specifically required in C′ fixation.
- Received July 8, 1953.
- Copyright © 1953 by The American Association of Immunologists, Inc.
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Studies on the Role of Ca++ and Mg++ in Complement Fixation and Immune Hemolysis
