Summary
The relative potencies of amboceptors may be determined with data from experiments planned in factorial design and evaluated through the analysis of variance. Dose-response lines are calculated from the percentages of lysis obtained in the presence of a constant amount of complement (1.2 C'H50 units) at three different levels of the unknown and of a standard amboceptor preparation in the range of the supplementary zone.
The method is illustrated with data from a typical assay and the results obtained with two more amboceptors are briefly reported.
Footnotes
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↵1 A preliminary presentation of this paper was given at the Fifth International Congress for Microbiology, Rio de Janeiro, Aug. 17–24, 1950.
- Received June 25, 1951.
- Copyright © 1952 by The American Association of Immunologists, Inc.
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