Summary and Conclusions
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1. The counting method of Backus and Williams is described as applied to enumeration of the elementary bodies of the agent of meningo-pneumonitis. The necessity of counting particles of biologic origin but of “indeterminate” nature, present in elementary body suspensions, is described.
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2. Homogeneity of the distribution of latex spheres, elementary bodies and other particles of biologic origin appears to be present in the results of this counting technic, at several concentrations of the elementary body suspensions.
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3. The estimates of concentration of biologic particles generally, or of elementary bodies alone, have a minimum standard deviation of about 3% resulting from instrumental limitations in determining diameter of latex spheres.
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4. Values for concentrations of elementary bodies, and of biologic particles generally, are reported, in which one standard deviation represents approximately 5% of the figure for concentration.
Footnotes
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↵1 Aided by grants from the National Foundation for Infantile Paralysis, and the Fluid Research Found of Yale University School of Medicine. Supported in part by a contract recommended to the Department of Medicine and Surgery, Veterans Administration, by the National Research Council. This project was conducted during the appointment of the senior author as Fellow in Virology of the National Foundation for Infantile Paralysis, and National Research Council Fellow in the Medical Sciences.
- Received January 21, 1952.
- Copyright © 1952 by The American Association of Immunologists, Inc.
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