Summary
Purified fractions of diphtherial toxin prepared under a variety of conditions have been examined electrophoretically and the Lf/mg N value of each fraction correlated with the area of the toxin peak. A minimum value of 2400–2600 Lf/mg N for electrophoretically homogeneous diphtherial toxin has been calculated.
The quantitative flocculation reaction has been performed on a purified fraction of diphtherial toxin. An immunochemical value in the neighborhood of 2500 Lf/mg N for diphtherial toxin has been calculated.
The precipitinogen titers against anti-Corynebacterium diphtheriae rabbit serum of purified diphtherial toxin fractions prepared under a variety of conditions have been compared. Fractions of Lf/mg N values approaching electrophoretic homogeneity were found to differ widely in their precipitinogen titers depending on the conditions of fractionation.
Footnotes
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↵1 Taken from a thesis submitted by Irwin H. Lepow in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
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↵2 A preliminary report of this investigation was presented March 17, 1950 before the Armed Forces Epidemiological Board, Commission on Immunization, U. S. Army, Washington, D. C.
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↵3 This investigation was supported in part by a research grant from the National Institutes of Health, Public Health Service.
- Received October 18, 1951.
- Copyright © 1952 by The American Association of Immunologists, Inc.
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