Summary
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1. An improved method for studying hemagglutination by Col-SK virus is described.
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2. A stoichiometric relationship is shown to exist between the amount of red cells and of hemagglutinin reacting at the endpoint.
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3. Human, rat and guinea pig erythrocytes agglutinate specifically with Col-SK virus in K-veronal buffer at 0 C.
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4. At 0 C 100% of the initial virus amount is adsorbed, at 20 C 88%, at 35 C 50%—at infinite time. There is no spontaneous elution. However, approximately 17% of the virus adsorbed at 0 C will elute at 35 C in veronal buffer.
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5. Hypertonic salt concentrations inhibit hemagglutination. Hemagglutination was inhibited by a comparatively slight increase in the Na concentration whereas a much more drastic increase in the K concentration is needed to produce the same effect. Both CaCl2 and MgCl2 inhibit hemagglutination above 0.01 M concentration. Adsorption proceeds at the same rate in CaCl2 as in NaCl.
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6. Evidence of identity of infectious agent and hemagglutinin includes data on: a) the amount of hemagglutinin and the infectivity titer of nervous tissue from different animal species, b) a definite correlation between hemagglutination inhibition and neutralization titer of specific immune sera, c) the similarity of physical and chemical properties of infectious and hemagglutinating principles.
Footnotes
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↵1 Aided by a grant from the Sister Elizabeth Kenny Foundation.
- Received December 4, 1951.
- Copyright © 1952 by The American Association of Immunologists, Inc.
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