Correction: Zebrafish F-box Protein fbxo3 Negatively Regulates Antiviral Response through Promoting K27-Linked Polyubiquitination of the Transcription Factors irf3 and irf7

Zhi Li; Sijia Fan; Jing Wang; Xiaoyun Chen; Qian Liao; Xing Liu; Gang Ouyang; Hong Cao; Wuhan Xiao

doi:10.4049/jimmunol.2001164

Li, Z., S. Fan, J. Wang, X. Chen, Q. Liao, X. Liu, G. Ouyang, H. Cao, and W. Xiao. 2020. Zebrafish F-box protein fbxo3 negatively regulates antiviral response through promoting K27-linked polyubiquitination of the transcription factors irf3 and irf7. J. Immunol. 205: 1897–1908.

Several of the occurrences of irf7 and irf3 in the legend for Fig. 9 were incorrect as originally published. The labels in the figure were correct.

FIGURE 9.

Overexpression of fbxo3 inhibits K27-linked polyubiquitination of irf3 and irf7. (A) fbxo3 promoted the polyubiquitination of irf7 but not lysine 48 (K48)-linked polyubiquitination and lysine 63 (K63)-linked polyubiquitination. (B) fbxo3 promoted lysine 27 (K27)-linked polyubiquitination of irf7 but did not promote K6-, K11-, K29- and K33-linked polyubiquitination. (C) fbxo3 promoted K27-linked polyubiquitination of irf7. (D) fbxo3 promoted the polyubiquitination of irf3 but not K48-linked polyubiquitination and K63-linked polyubiquitination. (E) fbxo3 promoted the K27 polyubiquitination of irf3 but did not promote the K6-, K11-, K29- and K33-linked polyubiquitination of irf3. (F) fbxo3 promoted K27-linked polyubiquitination of irf3. HEK 293T cells were transfected with HA-irf3 (6 μg) or HA-irf7 (6 μg), Myc-fbxo3 (4 μg), or empty vector (4 μg), together with His-ubiquitin or its mutants (4 μg). At 18 h posttransfection, the cells were treated with MG132 for 6 h. At 24 h posttransfection, the cells were lysed using guanidinium chloride, and purified with Ni²⁺-NTA agarose. IB, immunoblot; KO, K-only; KR, K is mutated to R.

In addition, the phrase “not promoted” in the legends for panels (B) and (E) was changed to “did not promote” for clarity and in the legend for panel (E), “K33 -inked” was changed to “K-33-linked.” Also, “Ni²-agarose” has been corrected to be “Ni²⁺-agarose” in the Materials and Methods section in the fourth line of the first paragraph under the heading “Ubiquitination assay” and in the legend for panel (F).

The corrected legend for Fig. 9 is shown below. The legend for Fig. 9 and the text have been corrected in the online version of the article, which now differs from the print version as originally published.