A Whole Blood Enzyme-Linked Immunospot Assay for Functional Immune Endotyping of Septic Patients

Monty B. Mazer; Charles C. Caldwell; Jodi Hanson; Daniel Mannion; Isaiah R. Turnbull; Anne Drewry; Dale Osborne; Andrew Walton; Tessa Blood; Lyle L. Moldawer; Scott Brakenridge; Kenneth E. Remy; Richard S. Hotchkiss

doi:10.4049/jimmunol.2001088

A Whole Blood Enzyme-Linked Immunospot Assay for Functional Immune Endotyping of Septic Patients

Monty B. Mazer, Charles C. Caldwell, Jodi Hanson, Daniel Mannion, Isaiah R. Turnbull, Anne Drewry, Dale Osborne, Andrew Walton, Tessa Blood, Lyle L. Moldawer, Scott Brakenridge, Kenneth E. Remy and Richard S. Hotchkiss

J Immunol January 1, 2021, 206 (1) 23-36; DOI: https://doi.org/10.4049/jimmunol.2001088

Monty B. Mazer

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

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Charles C. Caldwell

†Division of Research, Department of Surgery, University of Cincinnati, Cincinnati, OH 45267;

‡Division of Research, Shriner’s Hospital for Children—Cincinnati, Cincinnati, OH 45229;

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Jodi Hanson

§Cellular Technology, Shaker Heights, OH 44122;

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Daniel Mannion

¶Saint Louis University School of Medicine, St. Louis, MO 63104;

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Isaiah R. Turnbull

‖Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110;

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Anne Drewry

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

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Dale Osborne

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

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Andrew Walton

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

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Tessa Blood

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

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Lyle L. Moldawer

#Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610;

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Scott Brakenridge

#Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610;

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Kenneth E. Remy

**Division of Pediatric Critical Care, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110; and

††Division of Pulmonary and Critical Care, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110

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Richard S. Hotchkiss

*Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110;

‖Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110;

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The Journal of Immunology

Vol. 206, Issue 1

1 Jan 2021

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A Whole Blood Enzyme-Linked Immunospot Assay for Functional Immune Endotyping of Septic Patients