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In the flow cytometry histogram plot panel in Fig. 2F, in the first two panels (polyI:C) the naive mice were both Ifnar−/−, and in the second two panels (Vaccinia) the naive mice were both WT. This was a mistake. Although these data were collected at the same time and are presented next to the same controls for reference, the naive samples for the Ifnar−/− should have been placed in the Ifnar−/− panels, and the naive samples for the WT should have been placed in the WT panels.
PD-L1 is upregulated following type 1 IFN stimulus. (A) Representative 200× images and quantification of LNs from naive and day 3–5 vaccinia virus– and CHIKV-infected mice. Sections were stained for Lyve-1 and PD-L1. Fold increase in PD-L1 expression over naive is shown. Gates were drawn around the Lyve-1–positive staining, and the average PD-L1 geometric mean fluorescence intensity (gMFI) was taken and normalized to naive. Large image scale bar, 250 μM; inset image scale bar, 50 μM. (B) Representative plots and quantification of PD-L1 expression on LECs in the dLN following infection with vaccinia and CHIKV virus and immunization with poly I:C. The fold increase in PD-L1 gMFI compared with naive is shown. (C) Representative flow plots of PD-L1 expression on the dLN LECs following poly I:C immunization. (D) Quantification of (C) showing PD-L1 gMFI on all dLN LECs over time. (E) RT-qPCR showing fold increase in IFN-α4, IFN-β, and IFN-γ expression in the dLN 5 h postimmunization with poly I:C. Data are normalized to naive. (F) Representative flow plots of PD-L1 expression in WT and Ifnar−/− mice, 24 h postimmunization with poly I:C or 5 d postinfection with vaccinia virus. The fold increase in PD-L1 expression on LECs over naive is shown. (G) Quantification of (F). All experiments used two to three replicates per group and were repeated twice with similar results. Statistical analysis was done using an unpaired t test or one-way ANOVA. ***p < 0.001, ****p < 0.0001.
In the corrected version, we have placed the WT naive sample in the WT histograms and the Ifnar−/− naive sample in the Ifnar−/− histograms. The data and conclusions in the article are not affected. The figure legend was correct as published and is shown below for reference. The figure has also been corrected online, which now differs from the print version as originally published.
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