Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Genetic Variation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma

Alane Blythe C. Dy, Muhammad Z. Arif, Kenneth J. Addison, Loretta G. Que, Scott Boitano, Monica Kraft and Julie G. Ledford
J Immunol September 1, 2019, 203 (5) 1122-1130; DOI: https://doi.org/10.4049/jimmunol.1900546
Alane Blythe C. Dy
*Clinical Translational Sciences, University of Arizona Health Sciences, Tucson, AZ 85721;
†Asthma and Airway Disease Research Center, Tucson, AZ 85724;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Muhammad Z. Arif
‡Department of Medicine, University of Arizona, Tucson, AZ 85724;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kenneth J. Addison
†Asthma and Airway Disease Research Center, Tucson, AZ 85724;
§Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Loretta G. Que
¶Department of Medicine, Duke University School of Medicine, Durham, NC 27710; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Loretta G. Que
Scott Boitano
†Asthma and Airway Disease Research Center, Tucson, AZ 85724;
‖Department of Physiology, University of Arizona, Tucson, AZ 85724
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monica Kraft
†Asthma and Airway Disease Research Center, Tucson, AZ 85724;
‡Department of Medicine, University of Arizona, Tucson, AZ 85724;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie G. Ledford
†Asthma and Airway Disease Research Center, Tucson, AZ 85724;
§Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF
Loading

Article Figures & Data

Figures

  • Additional Files
  • FIGURE 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 1.

    Assessment of BALF eosinophilia over time. (A) OVA model of allergic airways. (B) Cell distribution in BALF at 24 h, 3 d, and 5 d after terminal challenge. (C) Net change in eosinophil frequencies over time. Table shows difference in means at 24 h and 5 d, unpaired Student t test. One-way ANOVA with Bonferroni correction for multiple comparisons, data (mean ± SEM) are from at least two independent experiments with n = 3–5 mice per group. *p < 0.05, **p < 0.01.

  • FIGURE 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 2.

    Assessment of tissue eosinophilia over time. (A) Representative bright field images of eosinophils (red arrows indicate representative eosinophils with bright pink–stained cytoplasm) in lung tissue by Sirius Red staining (left panel, original magnification ×40; right panel, original magnification ×100) and (B) quantification of eosinophil counts at day 5. *p < 0.05. (C) Net change in eosinophil frequencies over time. Table shows difference in means at 24 h and 5 d, unpaired Student t test, #p < 0.05, **p < 0.01. (D) EAR mRNA in lung tissue at 5 d after terminal challenge. One-way ANOVA with Bonferroni correction for multiple comparisons. Data (mean ± SEM) are from at least two independent experiments with n = 3–5 mice per group. *p < 0.05.

  • FIGURE 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 3.

    Examination of eotaxins in BALF after OVA challenge. Analysis of protein concentrations by ELISA of (A) eotaxin-1 or CCL11 and (B) eotaxin-2 or CCL24. One-way ANOVA with Bonferroni correction for multiple comparisons. Data (mean ± SEM) are from at least two independent experiments with n = 3–5 mice per group. **p < 0.01, ***p < 0.001, ***p < 0.0001 compared to untreated samples within each group.

  • FIGURE 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 4.

    Evaluation of the ability of SP-A to induce eosinophil migration in vitro. (A) Migration of mouse eosinophils was measured by a plate-based assay. Migration index calculated as number of live eosinophils in the bottom chamber over control. (B) SP-A concentrations in BALF of OVA-challenged mice. One-way ANOVA with Bonferroni correction for multiple comparisons. Data (mean ± SEM) are from at least two independent experiments, with n = 2–3 replicates per treatment or 3–5 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001. UT, untreated.

  • FIGURE 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 5.

    Evaluation of the ability of SP-A to induce eosinophil apoptosis in mouse and human eosinophils in vitro. (A) Time course of viability assessed by trypan blue and (B) Real-Time Cell Analyzer tracing and dose response of in vitro stimulation of mouse eosinophils by SP-A. (C) Representative flow diagrams of human eosinophil apoptosis and cell death by Annexin V and PI and quantification after 16 h incubation with SP-A. Live, Annexin V−, PI−; early apoptosis, Annexin V+, PI−; late apoptosis/dead, Annexin V+, PI+. (D) Densitometry of caspase-3 by Western blot of mouse eosinophils standardized to nontreated control. ANOVA with correction for multiple comparisons. Data (mean ± SEM) are from at least two independent experiments, with n = 2–3 replicates per treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

  • FIGURE 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    FIGURE 6.

    Evaluation of the effect of exogenous SP-A administration on eosinophils in SP-A–deficient mice after OVA challenge. (A) Schematic of OVA challenge and SP-A rescue. (B) Representative flow diagrams of eosinophil apoptosis and cell death by Annexin V and PI. (C) Total live eosinophil counts in BALF 5 d postterminal challenge. Data (mean ± SEM) are representative of two independent experiments with n = 5 mice per group. *p < 0.05.

Additional Files

  • Figures
  • Data Supplement

    • Supplemental Figures 1 (PDF)
PreviousNext
Back to top

In this issue

The Journal of Immunology: 203 (5)
The Journal of Immunology
Vol. 203, Issue 5
1 Sep 2019
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Genetic Variation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Genetic Variation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma
Alane Blythe C. Dy, Muhammad Z. Arif, Kenneth J. Addison, Loretta G. Que, Scott Boitano, Monica Kraft, Julie G. Ledford
The Journal of Immunology September 1, 2019, 203 (5) 1122-1130; DOI: 10.4049/jimmunol.1900546

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Genetic Variation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma
Alane Blythe C. Dy, Muhammad Z. Arif, Kenneth J. Addison, Loretta G. Que, Scott Boitano, Monica Kraft, Julie G. Ledford
The Journal of Immunology September 1, 2019, 203 (5) 1122-1130; DOI: 10.4049/jimmunol.1900546
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Disclosures
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • A Critical Role for Na+/H+ Exchanger Regulatory Factor 1 in Modulating FcεRI-Mediated Mast Cell Activation
  • PRMT1 Modulates Processing of Asthma-Related Primary MicroRNAs (Pri-miRNAs) into Mature miRNAs in Lung Epithelial Cells
  • A Rapid Shift from Chronic Hyperoxia to Normoxia Induces Systemic Anaphylaxis via Transient Receptor Potential Ankyrin 1 Channels on Mast Cells
Show more ALLERGY AND OTHER HYPERSENSITIVITIES

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606