Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Most Read
    • Top Downloads
    • Annual Meeting Abstracts
  • COVID-19/SARS/MERS Articles
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
    • Accessibility Statement
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Biologically Inspired, iterative engineering of a Human Lymphoid Follicle Chip

Girija Goyal, Jaclyn Long, Oren Levy and Donald E. Ingber
J Immunol May 1, 2018, 200 (1 Supplement) 120.34;
Girija Goyal
1Wyss Institute for Biologically Inspired Engineering at Harvard University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaclyn Long
1Wyss Institute for Biologically Inspired Engineering at Harvard University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Oren Levy
1Wyss Institute for Biologically Inspired Engineering at Harvard University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Donald E. Ingber
1Wyss Institute for Biologically Inspired Engineering at Harvard University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Organs Chips are microengineered, three-dimensional (3D) in vitro models that simulate organ-level pathophysiology and therapeutic responses with high fidelity. Here, we report the development of an in vitro model of lymphoid follicles seen in germinal centers and sites of chronic inflammation. The Human Lymphoid Follicle Chip was designed to mimic lymph flow through the lymph node as well as its cellular and matrix composition. A subcapsular sinus like channel allows the media to flow around the follicles with a fraction of it flowing through the parenchyma. We provide the first known evidence that 3D organization, cellular density and physical forces such as interstitial flow play a role in formation of tertiary lymphoid organs and germinal centers. Like tertiary lymphoid organs, CXCL3 production by lymphocytes is observed in the follicle chip concomitant with the aggregation of T and B cells. The follicles formed in vitro recapitulate the gross expansion and production of antibodies and cytokines seen in vivo in response to bacterial stimulation. While trying to incorporate vasculature into the follicle on chip, we found that our in vitro cultures mimic the killing of allogeneic endothelial cells, a clinically important phenotype in graft rejection that previously couldn’t be modeled without humanized mice.

Mice and conventional two-dimensional (2D) cultures of circulating human immune cells, even together, can fail to replicate human biology, leading to low efficacy and unpredicted, sometimes severe toxicity in clinical trials. In future experiments, the Follicle Chip will enable the assessment of candidate therapeutics, and specifically immunotherapies, in a patient-specific manner in vitro.

  • Copyright © 2018 by The American Association of Immunologists, Inc.
Previous
Back to top

In this issue

The Journal of Immunology
Vol. 200, Issue 1 Supplement
1 May 2018
  • Table of Contents
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Biologically Inspired, iterative engineering of a Human Lymphoid Follicle Chip
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Biologically Inspired, iterative engineering of a Human Lymphoid Follicle Chip
Girija Goyal, Jaclyn Long, Oren Levy, Donald E. Ingber
The Journal of Immunology May 1, 2018, 200 (1 Supplement) 120.34;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Biologically Inspired, iterative engineering of a Human Lymphoid Follicle Chip
Girija Goyal, Jaclyn Long, Oren Levy, Donald E. Ingber
The Journal of Immunology May 1, 2018, 200 (1 Supplement) 120.34;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Info & Metrics

Related Articles

Cited By...

More in this TOC Section

  • MHC clustering regulates selectivity and effectiveness of T-cell responses
  • Identifying antibodies and T-cell receptors with known specificity in repertoire sequencing data
  • Use of modified peptides for live tracking of peptide-MHCII complexes during antigenspecific T cell-dendritic cell interactions
Show more Technological Innovations I

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Accessibility Statement
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2021 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606