Ramachandran, M., C. Jin, D. Yu, F. Eriksson, and M. Essand. 2014. Vector-encoded Helicobacter pylori neutrophil-activating protein promotes maturation of dendritic cells with Th1 polarization and improved migration. J. Immunol. 193: 2287–2296.
The Western blot in Fig. 1B of the published article is not for the detection of Helicobacter pylori neutrophil-activating protein (HP-NAP) secretion by the Ad5[i/ppt-sNAP] virus, as stated in the figure and figure legend, but for the detection of Ad5PTDf35[Δ24-sNAP] virus, which is used in experiments for Figs. 6 and 7.
The Western blot for detection of HP-NAP secretion from Ad5PTDf35[Δ24-sNAP] virus has already been published (Ramachandran, M., D. Yu, A. Wanders, M. Essand, and F. Eriksson. 2013. An infection-enhanced oncolytic adenovirus secreting H. pylori neutrophil-activating protein with therapeutic effects on neuroendocrine tumors. Mol. Ther. 21: 2008–2018).
The correct Western blot for detection of HP-NAP expression by the Ad5[i/ppt-sNAP] virus is shown below. The band appears at the same expected molecular mass (15 kDa). There is no change in the figure legend or in the Materials and Methods. The figure legend published previously appears below for reference.
Illustration of adenoviral vectors used and Western blot showing secretion of HP-NAP. (A) An adenovirus serotype 5 vector was modified by replacing the E1 region with the HP-NAP transgene placed under the control of the H19-insulator–shielded, shortened prostate cell–specific recombinant promoter i/ppt and named Ad5[i/ppt-sNAP]. The HPNAP is His tagged at the N terminus and has an artificial leader sequence for efficient secretion. There are six copies of target sequence for the liver-specific microRNA miR122 in the 3′ untranslated region of the transgene. Ad5[Mock] was made by removing the E1 region. (B) Supernatants from LNCaP cells transduced with either Ad5[i/ppt-sNAP] or Ad5[Mock] were resolved by 10% SDS-PAGE gel electrophoresis, and secreted HP-NAP protein was detected by Western blot using an anti–HP-NAP Ab (clone 16F4, a gift of I. Iankov, Mayo Clinic, Rochester, MN).
- Copyright © 2015 by The American Association of Immunologists, Inc.
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